最近禽流感婴儿粪便中益生菌的分离筛选及培养

2020年11月4日发(作者：金彤)
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婴儿粪便中益生菌的分离筛选及培养

作者：郑丽君 吴秀英 李洪亮

来源：《农产品加工·上》2019年第04期

摘要：采用从婴儿 粪便中提取具有益生作用的菌种，为下一步的研究开发做乳酸菌种库做
积累。选取河南省洛阳市 5位志 愿者进行样品采集，得到5份完全由母乳喂养的婴儿（2月
龄）自然排出的粪便样，并分离纯化出5株乳 酸菌（RS1， RS2，RS3，RS4，RS5）和5株双
歧杆菌（SQ1，SQ2，SQ3，SQ 4，SQ5），经鉴定，确定RS3和SQ1具有益生作用的。结果
表明，此2株菌具有较好的耐酸性、 胆盐耐受力，对温度敏感，对模拟胃液和肠液具有较强的
耐受力，且对大肠杆菌、沙门氏菌具有95%以 上的抑菌率，可作为优良的益生菌候选菌种。
关键词：婴儿粪便；乳酸菌；双歧杆菌；分离鉴定
中图分类号：S852.6 文献标志码：A doi：.1671-9646（X）.2019.04.003
Isolation and Cultivation of Probiotics from Infant Faeces
ZHENG Lijun，WU Xiuying，LI Hongliang，SUN Tao
（Inner Mongolia Mengniu Dairy Industay（Group）Co.，Ltd，Huhot，Inner Mongolia
011517，China）
Abstract：Along with increased recognition of the probiotics， however， the basic mechanism
of probiotics hasn't been completely unified up to now，which needs further research and analysis.
The purpose of this study was to isolate probiotic strains from the feces of infants，and to lay the
foundation for constructing the library of probiotics next step. Five natural excrement samples were
collected from full breastfeeding babies（2 months）of Luoyang He'nan，five Lactic acid bacteria
（RS1，RS2，RS3，RS4，RS5）and five Bifidobacterium（SQ1，SQ2，SQ3，SQ4，SQ5）
were isolated after purification. Furthermore，two strains（RS3，SQ1）were characterized to have
probiotic function. The results showed that these two strains were well resistant to acid and bile
condition，sensitive to temperature，and highly tolerant to simulated gastric and intestinal fluid. In
addition，up to 95% E. Coli and Salmonella were inhibited by these two strains. Based on their
phenotypic characteristics，（RS3）and（SQ1）can be excellent candidates of probiotics library.
Key words：baby faeces；Lactobacillus；Bifidobacterium，；isolation and identification
0 引言
益生 菌是一類可以对宿主细胞起到有益作用的活性微生物，定植在人体肠道和生殖系统中
[1]，分为乳酸菌 类、双歧杆菌类、革兰氏阳性双歧杆菌。研究显示，人体中微生物种类约30